Summer Research Report - 804 Words

The first weeks of the summer fellowship in the VDS stream was a bit hectic. They consisted of learning protocols and techniques. Three plates of PNiC-BSa4 colonies were grown by my partner and me that week. These were later used by the majority of lab students who had reached the cloning stage. My first PCR with plasmid pgbr22 failed, but the second was successful. PCR with pmCherry had to be completed 3 times to get a successful result and PNIC-BSa4 PCR had to be completed twice to get a successful result. The three practice PCRs were completed the 5th week of lab. Protein expression using PfDXR was started in week 3 and the sample of protein was purified the following week. I received my target protein Rickettsia prowazskii FabG†¦show more content†¦The PCR2 sample from the previous week was purified through PCR clean up and nano-dropped. The concentration was 68ng/ul. The concentration of this sample was low (lt;100ng/ul) because I had skipped the first step in the cle anup process that prepares the column filter for maximum DNA binding. PCR2 and PCR clean up were completed again and the concentration of the sample was averaged to be 147.9ng/ul. Since the concentrations of the samples were adequate, I moved onto PNiC-BSa4 cloning and prepared a sample to be DNA sequenced. RE digest was completed on two PNIcBSa4 prep samples, with the gel indicating that both samples were successfully cut and nano-drop results indicating high purity levels with low concentrations (~32ng/uL). Results from sequencing were nucleotide blasted and confirmed to be PNiCBSa4. I talked to a mentor about my samples and was told that I probably needed a higher concentration ≠¥100ng/uL, for cloning to be successful. In order to increase the concentration 4 samples of 50uL each were cut and PCR clean up into one sample that had a concentration of 123.5ng/ul. The cloning protocol was completed that week and when the plated were checked, no colonies were present on either of the two plates. My first attempt at cloning failed. The process was completed again, and the sample concentration was averaged to be 112.1ng/ul. The second time, only oneShow MoreRelatedThe Year Round Schooling System Essay947 Words   |  4 Pagesstems from the original agrarian calendar as students were often called to tend to crops in the summer. This was thoughtfully and effectively designed for the time period it was created in; however, schools, research, and society have dramatically changed since then. The agrarian calendar no longer makes sense, as we consider overcrowding in schools and the significant research regarding the â€Å"summer brain drain.† There are several models of year-round schooling that would better serve all stakeholdersRead MoreLarry Bird1512 Words   |  7 Pageswill be in its 15th annual cycle during Summer 2002 and is designed to attract students to various fields of clinical research. Students also gain valuable experience assessing validity of current clinical practice standards in a discipline. Research methods and data analysis aSTUDENT RESEARCH FELLOWSHIP PROGRAM This program will be in its 15th annual cycle during Summer 2002 and is designed to attract students to various fields of clinical research. Students also gain valuable experienceRead MoreDeposit Mobilization in Banks1071 Words   |  5 Pages[pic] School of Management [pic] SUMMER INTERNSHIP PROJECT On Deposit Mobilization A Research Project Submitted to Add Value in the Degree of Masters of Business Administration (2009-2011) Faculty Guide Company Guide Name Name Prof.Aditya Verma Mr. Ramesh singh Read MoreRole Of Sustainability On The Production Of The Firm s Healthy Bars1294 Words   |  6 PagesExecutive summary 2.1 The Purpose The purpose of this report is to highlight the roles of sustainability in the production of the firm s healthy bars. This report is provided to the chief executive in order to suggest few solutions to critical issues including high electricity bills, large consumption of water in the production process; and advantages and disadvantages for each solution. 2.2 Methodology The methods used In this report was form finding informations by researching books andRead MoreCase Study : The Summer Student Program1017 Words   |  5 Pages What’s SSP All About? The Summer Student Program is designed for students who want to immerse themselves in genetics and genomics research. It emphasizes laboratory discovery, communication of knowledge, and professional growth. Students participate in an ongoing research program with the support of an experienced scientific mentor. They develop an independent project, implement their plan, analyze the data, and report the results. At the end of the summer, they present their findings to researchersRead MoreYear Round Schooling Is Better Than Traditional Schooling1599 Words   |  7 PagesYear round schooling is better than traditional schooling because, the summer retention is much higher, special education and english language learners benefit much more, end of the year test scores are higher and the year round school calendar fits our culture more now than it used to with the traditional school year. As an education major with a focus in special education, I was trying to figure out what to write and research for this paper. Starting to look at the the topics list at the bottomRead MoreClimate Change in New Zealand1682 Words   |  7 Pagesdue to the agricultural industry being unable to cope with the conditions. Citizen health will decline in the next hundred years with warmer summers bringing an increased risk of air-borne and water-borne diseases. 2 Table of Contents Abstract 1 1.Introduction. 3 2.Findings. 4 Main Research Question: How might climate change affect New Zealand? 2.1 What are the predicted environmental impacts of climate change within New ZealandRead MoreThe Importance Of Volunteering In The Community1074 Words   |  5 Pagesdifference. Giving up a few hours during the week to volunteer at a summer reading program at the community library can bring joy in some child’s life. Supporting and helping younger children learn in our community brings me pride. Volunteering is a way to give back to your community and gain work experience at the same time. On Thursdays from 6:00-8:00, my sister and I volunteer at â€Å"Norther Tier Library† supporting the summer reading program. The program involves children from birth to 18 yearsRead MoreImproving The Corporate Team And Dine Out Team898 Words   |  4 Pagesbulk of the research requests independently during the summer while still completing my other daily tasks and backup assigned tasks. Frequently I pull online donation reports from Convio that require manipulation and proper coding before they are imported into Rasier’s Edge. Utilizing critical independent thinking, I am able to determine the proper coding for the gifts and then successfully import them into Rasier’s Edge. Because of my diligence with coding, I am able to pull reports that successfullyRead MoreReasons For Delaying Summer Born Children1410 Words   |  6 Pagesage. Children born in the summer months can be up to nearly a year younger than their autumn born counterparts. At aged four, this potential year significantly compromises a fraction of the children’s lives and therefore means summer born children may be behind in terms of development hindering their academic ability in comparison to their older counterparts. Whilst there is ongoing research into the topic, presently there are many conflicting views on whether delaying summer born children’s entrance